The Chief Ant is shown to be a large red ant wearing a blue military uniform adorned with three medals. He is also seen wearing a matching blue military hat.
After all the Ants had deliver the sugar for tonight dinner, The Chief Ant encounters The Lone Ant, Who brought home a soy bean. The Chieft Ant told him that Ant love sugar and force Lone Ant to like it. However when Lone Ant rejected the sugar, The Chief Ant was so enraged that he kicked Lone out of the anthill. Later he and the other Ants began searching for more sugar, up to the point where they had found a chocolate factory and began to eat the chocolate. Unfortunately he and the others were captured by the Chocolate Factory Manager, who plans to make them into chocolate covered ants. Just as he and the other were going into their doom, Lone Ant appeared and saved them by tossing lifesavers (candy) into the vat of chocolate. Afterwards The Chief Ant welcome back Lone Ant to the AntHill. Later that day when Lone Ant was giving all of the other Ants some healthy food, The Chief Ant tried to sneak in sugar, only to have Lone Ant stop him. He then tried a bite and began to eat the whole thing.
- He is the only ant to be able to talk.