Knuckles appears in the "Here's Hamton" episode segment "America's Least Wanted". Plucky has invited himself over to Hamton's house, and while he is channel flipping, he watches America's Top 10 Criminals, where he finds out Knuckles is currently public enemy #1, and that he is most likely living nearby under an assumed name. Due to Knuckles' resemblance to Hamton, Plucky is worried he might have been best friends with a master criminal.
When he hears that a $25,000 reward is offered, he becomes interested in turning Hamton in. He dresses Hamton in a black and white striped suit and a ball-and-chain, all the while worried he might be Knuckles. When he sees what looks like him choking Elmer Fudd, he tries to save Elmer. Turns out Hamton was rearranging Elmer's sacroiliac, as his regular chiropractor was on vacation. Plucky puts Hamton in a bag and decides to turn him over to the police.
On his way over, he bumps into a security guard loading bags of money into the bank. He misplaces the bag with Hamton inside and accidentally takes a bag of money. When he turns it over to the police, he gets arrested. Hamton pays his bail, but once he is free, Plucky tries to turn him over to the police. The police are not fooled, as they arrested the real Knuckles Cutlet almost an hour ago. Plucky, still desperate for the $25,000 reward, tries to turn Knuckles in, but he is booted out, along with Knuckles by accident. Hamton is arrested as Plucky takes Knuckles to Hamton's house. Plucky decides to trust his friends from now on, unaware that he was leading Knuckles to Hamton's house, and Knuckles tries to knock him out.
- Knuckles Cutlet is not to be confused with Knuckles the Echidna, a character from the Sonic the Hedgehog video game series. In fact, he predates him by several years.
- Let me go you screws I want my lawyer back.
- (After seeing Hamton dressed as a criminal) "Hey, who's that handsome guy in the stripes?"
- "Here's Johnny!"
- It makes it easier for me to sneak up on ya!