Big Daddy Boo is one of the first antagonists of Tiny Toon Adventures: How I Spent My Vacation. He is the father of the Boo Sisters, and intends for Buster Bunny to marry all three of them. Buster is able to narrowly avoid this terrible predicament when Babs interferes and outwits the troublesome gators, but not before she "enjoys watching him sweat." The bunnies later encounter them on the cruise ship while they are performing, as part of their carnivorous audience. He is voiced by Sorrell Booke in his last role.